This hands-on workshop on offers a deep technical dive into the Next-Generation Sequencing (NGS) and RNA-Seq data analysis, starting with Linux-based computational fundamentals and advancing through NGS data processing, gene expression quantification, and differential expression analysis. Participants will be trained to execute complete RNA-Seg analysis pipelines, from raw sequencing reads to biologically meaningful insights, using widely adopted tools such as HISAT2, StringTie, and DESeq2.

The workshop further emphasizes statistical analysis and data visualization using R, enabling participants to generate and interpret publication-quality graphical outputs, including heatmaps and volcano plots. In addition, a dedicated session will introduce fundamental biostatistics concepts and Python-based data analysis, employing libraries such as Biopython and pandas to develop robust, reproducible, and scalable computational workflows for NGS-based gene expression studies.

Dr. Pallavi Mishra (MUJ, Jaipur)   |   Dr. Akshay Kumar (IIT-Bomaby)   |   Mohd. Atique Barudgar (Techknowgreen Solutions)